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Wednesday, September 10, 2008

Web Source of PCR (1)

Legal Life Experience Degrees Get an Accredited & Verifiable US Biochemistry and Molecular Biology degree from home. Free Instant Delivery via Dhl. No study, no exams. Read Testimonials. www.belforduniversity.org (sponsored link) 3' RACE PCR This technique is used to obtain the 3''end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK.http://www.nottingham.ac.uk/~mbzspd/m...
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Adjuvants in PCR Reactions Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.http://info.med.yale.edu/genetics/war...
Alu-PCR Hybridization A report and protocol, from Fondation Jean Dausset.http://www.cephb.fr/manips_alupcr.html
Anchor Probes for Comparative Mapping of Grass Species Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.http://greengenes.cit.cornell.edu/anc...
Attotron Biosensor Corporation R & D company for development of biosensors and related products for the research and educational markets.http://www.attotron.com
Biosource molecular methods booklet 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).http://www.biosource.com/content/tech...
C. elegans Gene Knockout Project Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/
Cleaved Amplified Polymorphic Sequence Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.http://www.fsl.orst.edu/tgerc/PCRCour...
DNALC: PCR Animation An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.http://www.dnalc.org/shockwave/pcranw...
Degenerate PCR An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.http://cgat.ukm.my/protease/degpcr.html
Design Oligonunucleotide Primers Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.http://sciborg.uwaterloo.ca/~mpalmer/...
Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.http://imd-www.es.hokudai.ac.jp/~kinj...
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.http://www-ang.kfunigraz.ac.at/~binde...
Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Effect of PCR Buffer on multiplex PCR (PDF) Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)http://www.qiagen.com/literature/broc...
FISH Guide and Troubleshooting Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.http://info.med.yale.edu/genetics/war...
Fidelity of DNA Polymerases for PCR Article by PN Hengen from TIBS 1995http://www.lecb.ncifcrf.gov/~pnh/pape...
GenHunter Manufacturer of material for differential display PCR in Nashville, Tenn USA.http://www.genhunter.com
HiFi DNA HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.http://www.hifidna.com/
Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.http://www.btc-bti.com/pcrelisa.htm
Innovative Tools for Amplification Notes about PCR applications, principles, research results, from Roche.http://www.roche-applied-science.com/...

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