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Wednesday, September 10, 2008

Colony PCR Protocols

Colony PCR protocol
Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/colony_pcr.html

Colony PCRColony PCR. This protocol is designed to quickly screen for plasmid inserts directly from E. coli. colonies. The plasmid should be high copy number such as ...www.csun.edu/~mls42367/Protocols/Colony%20PCR.pdf

Colony PCR ProtocolColony PCR Protocol. 1. Pull out eight glycerol stock plates from the –80. o. C freezer and set on bench top to. thaw. Be sure to remove the foil seal ...microarrays.berkeley.edu/file_download.php?fid=13

Colony PCR ProtocolColony PCR Protocol. Overnight Culture 5.0µl. 10x PCR buffer 5.0µl. MgCl2 (25mM) 5.0µl. Forward primer (10µM) 0.2µl. Reverse primer (10µM) 0.2µl ...www.unc.edu/~fconlon/Protocols/Colony%20PCR%20Protocol.doc

Colony PCR
Colony PCR Protocol contributed by Lynn Hancock 1. Choose a reasonable sized colony (2-3 mm in diameter) and resuspend 100 µl ddH2O. 2. For PCR, ...www.enterococcus.ouhsc.edu/ColonyPCRProtocol.asp

Yeast Colony PCR -- Amberg et al.
Search CSH Protocols. Advanced Search · Find Protocols · Find a Kit · Access Personal Page · Submit Protocols · Help · Subscribe · About CSH Protocols ...www.cshprotocols.org/cgi/content/short/2006/1/pdb.prot4170

Colony PCR - OpenWetWare
From OpenWetWare. Jump to: navigation, search. See Colony PCR for general information about this protocol and other variants ...openwetware.org/wiki/Endy:Colony_PCR

Colony PCR protocol
Monserate Biotechnology Group Custom services, Colony PCR protocol.www.monseratebiotech.com/colony-pcr.html

Yeast Colony PCR protocolYeast Colony PCR protocol. derived from http://sequence-. www.stanford.edu/group/yeast_deletion_project/deletions3.html.

Colony PCR (Forsburg Lab)
The Forsburg lab pages: fission yeast colony PCR protocol. ... Colony PCR can be used to identify colonies where your favorite gene yfg1 has been replaced ...http://www-rcf.usc.edu/~forsburg/pcr.html

Rapid Screening by Direct Colony PCR Using the FastStart PCR Master
The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can be analyzed directly without prior template purification, ...www.analytica-world.com/articles/e/65346/

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