Variants of PCR (4)

PCR animated - Animation illustrating the principle of PCR, from the University of Ghent, Belgium.

PCR Gateway - A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.

PCR Guru - A downloadable textbook on PCR setup and optimization, not free.

PCR method protocols - Protocols for PCR posted by the Helen Donis-Keller Laboratory.

PCR primer design and reaction optimisation - Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.

PCR Project - Presentations from the University of California at Berkley on PCR, both current research reports and reviews.

PCR Protocol - Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.

PCR Protocols - Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University

PCR Technology - An introduction by Connie Veilleux from the US National Health Museum website.

PCR Troubleshooting - Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.

PCR-ELISA and Related - P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA.

Polymerase Chain Reaction - Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.

Polymerase Chain Reaction (PCR) - A graphic description of the principle of PCR from the US National Health Museum web site.

Principle of PCR - Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.

Protocols Online: PCR Protocols - Extensive collection of PCR protocols and methods from Protocol On Line.


Quantitative PCR Protocol - From the Jackson Laboratory, University of Maine, USA.

Random Arbitrarily Primed PCR - Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.

RAPD analysis with P. infestans - Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.

RAPD PCR - RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.

RAPD's - Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.

Rational primer design greatly improves differential display-PCR (DD-PCR) - Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

Reference in PCR - Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.

Rep-PCR Genomic Fingerprinting - Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.

RFLP Definition - RFLP = Restriction Fragment Length Polymorphism, from FDA

Roe Laboratory Protocols - Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.

Single tube confirmation PCR protocol - For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.

Standard PCR protocols - From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.

Tavi's PCR protocols - A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.

T-DNA Generated Enhancer Traps in Arabidopsis - Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.

Technical manual of PCR - Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.

The "Poison Primer" Technique for Enhancing Detection of Small Deletions in Mutant Libraries - This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.

The web guide of PCR - List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.

Thermostable DNA Polymerases - Discussion of their origin and briefly their properties. From the web site of Colorado State University.

Wayward PCR primers - Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.

What the Heck is PCR? - Popular description of the PCR technique by John C Brown, University of Kansas 1995.

Which DNA Marker for Which Purpose? - Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen.