Alternate method for purifying DNA from agarose gels
Isolation of RNA from mammalian cells using RNAZOL (Teltest)
Preparation of sequencing gels
Preparing proteins/antibodies/DNA/RNA for microinjection
Safety Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment
Transfection of mammalian cells using Lipofectamine (LTI)
Transformation of E. coli by electroporation
Additional Applications of Confocal Microscopy
Comparison of Electron Micrographs
Confocal Microscopy (Information about the history, confocal theory, principles and optics as well as a selection of general links)
Conventional VS Laser scanning Microscopy Techniques
Creatingand enhancing professional graphics using Adobe PhotoshopCS3
Dark field illumination, Larry Legg
Digital Image Ethics (A short history of some of the issues that journalists have struggled with and ten suggested guidelines for working with digital images)
Digital Microscope Camera Technology
Confocal Laser Scanning Microscopy
Light Microscope Techniques
Materials, Forensics, Geological Microscopy
Electron and Light Microscopy Web Resources
Educational Resources (On-line resources for K-12 teachers & students)
Electron Microscope Techniques
Historical and Antique Microscopes
Scanning Probe and Atomic Force
Energy Dispersive Microanalysis EDX
Electron Microscopy (Information about history, principles, sample preparation techniques and www resources for TEM & SEM)
Fluorescence Techniques (Links with information about fluorescent dyes, antibodies and resources for sample preparation)
Free Publications (Seven publications for professionals that cover microscopy & imaging from a variety of perspectives)
Hardware (Information about digital cameras, scanners and printers)
Histology (Links to sample preparation protocols and staining techniques, immuno/histo/cyto-chemistry, collections of images of tissues prepared for histology and safety)
Image Galleries (Links to collections of beautiful images that use a number of microscopic imaging techniques0
Introduction (A look at the basics of what makes up a digital image, color and file formats, as well as a few links on human vision)
Laser microscopy: stereo-viewer
Light & Microscopes Larry Legg's funky introduction
Light Microscopy (Links to excellent resources that introduce concepts of light & optics, as well as how light microscopes work)
McCrone Research Institute Teaching light microscopy, pollen and spore identification
Measurement of Cell Organelles
Measurements: Ocular and Stage Micrometers
Microscopy - Wikipedia, the free encyclopedia
Microscopy Resources (General information about professional societies, vendors, web resources and email discussion groups)
Microscopy-UK general resource, training, and supplies
Phase Contrast illumination and other methods, Larry Legg
Polarized light illumination - Do-it-yourself, cheap
Polarized Light illumination, David Walker
Sample Preparation of Confocal Microscopy
Software (Links to resources for several commercial and freeware image editing programs)
The Transmission Electron Microscope
Video microscopy (Microscopy & Imaging Resources on the WWW)
Alkaline Phosphatase Localization
Cell fixation - room temperature paraformaldehyde (good for paraspeckles/speckles for 5 min; most other things for 10 min)
Cell fixation - methanol
Conjugating antibodies to fluorescent probes
Immunoflourescence of MEFs
Mouse Necropsy
Necropsy form
Paraffin embedding and Sectioning
Staining Protocols
H & E staining
BrdU Staining Histological Sections
X-gal Staining
HRP-DAB Immunostaining
AP-BCIP/NBT Immunostaining
GFP & BrdU In Situ Immunoflourescence
Fixation, permeabilization and immunostaining of cells (contains images and movies)
Cell fixation - warm paraformaldehyde (preserves microtubules)
Immunofluorescent Localization of Tubulin
Methyl Green-Pyronin Staining of DNA and RNA
Periodic Acid Schiff (PAS) Reaction
Affinity column preparation and Immunoprecipitation
Affinity purification of Antibodies
B-PER Bacterial Protein extraction reagent (Pierce)
Bradford quantification of protein concentration
Crosslinking the antibody to the beads protocol | Abcam
Factor Xa Cleavage of GST fusions
GST-fusion protein injection into rabbits
GST-protein fusion expression, purification and other uses
His-fusion protein purification (Novagen/Qiagen)
Histone Kinase Assay
Western blot
p27 ELISA
Protein Interactions Captured by Chemical Cross-linking: One-Step Cross Linking.
Protein preparation (from plants tissue)
Protocol: Cross-linking of IgG to Protein A or G Beads: NEB
Cell Fractionation Protocol.
Chromatin isolation
Spliceosome purification
Cell Fractionation Protocol.
Covalently conjugate antibodies to beads for affinity purification
Nucleolar isolation (contains images and movies)
Cajal body isolation (contains images and movies)
Lysing cells for release of both cytoplasmic and nuclear proteins
Equilibrium Density Gradient - Percoll
Preparation of nuclear extract from plated cells
Separation of Cells by Velocity Sedimentation
Amino-allyl labeling
BioAnalyzer
TRIzol plus RNEasy Mini
RNEasy Midi
TRIzol product insert
RNA amplification
RNA gel electrophoresis
C. elegans RNA prep: Michael Koelle
In vitro transcription reaction
Northern Blots: Michael Koelle
Phenol extraction of rRNA (rat liver)
RNA and protein extraction from the same sample
RNA prepararion (from plants tissue)
RNA Preparation: MGH
ABC Random Amplification
Alcohol Precipitation of DNA
AML sample DNA preparation
Boomerang DNA Amplification Single primer DNA amplification
cDNA amplification from l-phage library
DIG DNA labelling and hybrid detection (Boehringer Mannheim)
Direct Automated Sequencing off Bacterial Genomic DNABruce Rhoe
DNA extraction from agarose (Qiagen)
DNA - dische diphenylamine determination
DNA Fragment Isolation from LM agarose
DNA detection
DAPI chromosome identification
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Extraction of DNA from bovine spleen
Large Scale Plasmid Preparation: MGH
PCR product purification (Qiagen)
Perfectly Blunt Cloning of DNA (Novagen)
Phenol/chloroform extraction of DNA
Plasmid midi-prep from bacteria
Preparation of genomic DNA from bacteria
Random Primed labeling of DNA (Prime-It II -Stratagene)
Restriction enzyme digestion of DNA
Southern Hybridization
Stark's Prehybridization Buffer
Wizard Plus Mini-preps (109. Plasmid Mini-prep)
Worm genomic Southern blots: Michael Koelle
Competitive and/or Quantitative RT-PCR
History of Polymerase chain reaction (PCR)--(1)
History of Polymerase chain reaction (PCR)--(2)
Important Publications at Cell
Important Publications at Nature
Important Publications at Science
Ligation Mediated Suppression PCR
Long PCR Protocol: Church Lab
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