The method was originally developed by von Kossa, and underwent several modifications. The stain principle is a precipitation reaction in which silver ions react with phosphate (not calcium) in the presence of acidic material. Photochemical degradation of silver phosphate to silver then occurs under light illumination.
Additional methods need to be employed to confirm the presence of calcium and hydroxyapatite, such as Alizarin Red, which detects calcium deposits.
VON KOSSA Stain Procedure:
1. Sections to water.
2. Place sections in 1% silver nitrate solution under reasonable light for 30-60 minutes.
3. Wash in distilled water.
4. Treat with 3% sodium thiosulphate for 3-5 minutes.
5. Wash in water.
6. Counterstain with van Gieson for 3-5 minutes.
7. Wash in alcohol.
8. Clear .
RESULTS:
Mineralised bone black Osteoid red
Other Protols for Von Kossa
von Kossa Staining Protocol for Calcium
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www.ihcworld.com/_protocols/special_stains/von_kossa.htm
Von Kossa Stain - Wikipedia, the free encyclopedia
Von Kossa Stain. From Wikipedia, the free encyclopedia. Jump to: navigation, search. Thevon Kossa stain is used to quantify mineralization in cell culture ...
en.wikipedia.org/wiki/Von_Kossa_Stain
Von Kossa's Method - Calcium
VON KOSSA'S METHOD - CALCIUM. PURPOSE: Abnormal deposits of calcium may be found in any area of the body. With the H&E stain, calcium appear deep ...
library.med.utah.edu/WebPath/.../VONKOSSA.PDF
Renal Fellow Network: the von Kossa Stain for Acute Phosphate ...
17 Aug 2009 ... A good detection method is the use of the von Kossa stain, which stains certain calcium-containing salts such as calcium phosphate a ...
renalfellow.blogspot.com/.../von-kossa-stain-for-acute-phosphate.html
Technical Bulletin #444 von Kossa Staining of Osteoclast ...
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von Kossa staining alone is not sufficient to confirm that ...
Numerous techniques are currently used to characterize biological mineralization in intact tissues and cell cultures; the von Kossa staining method, ...
www.ncbi.nlm.nih.gov/pubmed/12724828
The von Kossa reaction for calcium deposits: silver lactate ...
We have demonstrated that positive von Kossa stain- ing correlates well with calcifications in... cultures the von Kossa stain usually yielded identifiable ...
www.springerlink.com/index/k782p815tn252t39.pdf
Re: [Histonet] von kossa stain
27 Jan 2004 ... My understanding of the von Kossa stain is that it is NOT specific for calcium. Rather, the silver nitrate in the staining solution (silver ...
www.histosearch.com/histonet/.../Re.Histonetvonkossastain.html
VON KOSSA STAIN KIT PROCEDURE
VON KOSSA STAIN KIT PROCEDURE. Item# KT 028. KIT. 100ml. Nuclear Fast Red Stain. 100ml. 5% Silver Nitrate. COMPONENTS. 100ml. 5% Sodium Thiosulfate ...
search.cosmobio.co.jp/cosmo_search_p/..._/KT028.20060119.pdf
von Kossa stain definition from the mondofacto online medical dictionary.
www.mondofacto.com/facts/dictionary?von+Kossa+stain
Von Kossa Staining Alone Is Not Sufficient to Confirm that ...
and cell cultures; the von Kossa staining method, elec- tron microscopic analysis (EM), ....However, the von Kossa stain is being used with greater ...
www.springerlink.com/index/TWUFXJY1VTHUVYR5.pdf
von Kossa and Alcian Blue Staining Protocol for Calcium and ...
Combination of von Kossa and Alcian Blue staining will make both calcium and mucosubstances visible on the same tissue sections. ...
www.ihcworld.com/_...stains/von_kossa.alcian_blue.htm
Histotechnology Technical methods. Stain for calcium ... VON KOSSA. 1. Sections to water. 2. Place sections in 1% silver nitrate solution under ultra- ...
www.nottingham.ac.uk/pathology/protocols/vonkoss.html
Von kossa stain: Definition with Von kossa stain Pictures and Photos
Definition of Von kossa stain with photos and pictures, translations, sample usage, and additional links for more information.
www.lexic.us/definition-of/von_kossa_stain
Stain Expert™ - Your Stains & Reagents Information Center
Calcium Stain - Von Kossa. Celestine Blue Method - Lendrum. Copper Stain (Rubeanic Acid). Cresyl Violet Acetate Method for Helicobacter sp. ...
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JoVE: Undecalcified Bone Preparation for Histology ...
8 Jan 2010 ... An identical protocol or rack staining is necessary for ... Von Kossa. Place in silver nitrate solution and expose to strong light until ...
www.jove.com/index/details.stp?id=1707
Isolation of human multipotent mesenchymal stem cells from second ...
We developed a novel two‐stage culture protocol for isolating MSCs from amniotic .... (d) Osteogenic differentiation was demonstrated by von Kossa staining. ...
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30 May 2007 ... Von Kossa staining of calcium-phosphate deposits was performed. ... (1999) [40] and a slightly modified protocol of the one reported by ...
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Fwd: [Histonet] Question on Von Kossa
Of course, as with any silver stain use acid cleaned glassware and gloves ... Does anyone have a good Von Kossa stain protocol that they would not mind ...
www.mail-archive.com/histonet@lists...edu/msg00672.html
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