AFLP PCR

What’s AFLP?-- From Wikipedia, the free encyclopedia
Amplified fragment length polymorphism PCR (or AFLP-PCR or just AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990’s by Keygene, AFLP uses restriction enzymes to cut genomic DNA, followed by ligation of complementary double stranded adaptors to the ends of the restriction fragments. A subset of the restriction fragments are then amplified using two primers complementary to the adaptor and restriction site fragments. The fragments are visualized on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies.

AFLP-PCR is a highly sensitive method for detecting polymorphisms in DNA. The technique was originally described by Vos and Zabeau in 1993. The procedure of this technique is divided into three steps:

1. Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.

2. Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences.

3. Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.
A variation on AFLP is cDNA-AFLP, which is used to quantify differences in gene expression levels.
Another variation on AFLP is TE Display, used to detect transposable element mobility.

AFLP
AFLP protocolAFLP protocolAFLP TechnologiesAFLP: principles and applications
AFLPs on the ABI 3100Amplified Fragment Length Polymorphism ProtocolStandard List for AFLP Primer NomenclatureAFLP: not only for fingerprinting, but for positional cloningAFLP PCR

In silico AFLP-PCR amplification

AFLP: not only for Fingerprinting, but for Positional Cloning

GE Healthcare Life Sciences - AFLP

AFLP (Liscum Lab.)

A PCR-based DNA fingerprinting technique: AFLP.

cDNA-AFLP Protocol

AFLP Protocol

References:

AFLP vs PCR-RFLP
AFLP Protocol Using Li-Cor Sequencer
http://www.oznet.ksu.edu/wheatgenotyping/aflp.html

AFLP
Selected links about AFLP. ... Amplified Fragment Length Polymorphisms and Microsatellites: A phylogenetic perspective This paper reviews the techniques of ...
www.pcrlinks.com/variants/aflp.htm


Amplified Fragment Length Polymorphism World of Genetics
Amplified Fragment Length Polymorphism World of Genetics. Amplified Fragment Length Polymorphism summary with 1 pages of encyclopedia entries, ...
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Fluorescent Amplified Fragment Length Polymorphism Analysis of ...
Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus and Bacillus thuringiensis Soil Isolates. Ticknor LO, Kolst AB, ...
www.ncbi.nlm.nih.gov/pubmed/11571195




Fluorescent amplified fragment length polymorphism analysis of ...
Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. ...
www.ncbi.nlm.nih.gov/pubmed/14766590




Google Directory - Science > Biology > Biochemistry and Molecular ...
A description of the Amplified Fragment Length Polymorphism technique of DNA fingerprinting. By U Melcher, Oklahoma State University, USA. ...
www.google.com/.../Science/.../Biochemistry_and_Molecular_Biology/.../PCR/....




Genetic Analysis - Amplified Fragment Length Polymorphism
Amplified Fragment Length Polymorphism (AFLP) analysis is one of the most powerful DNA fingerprinting methodologies for the identification and typing of ...
www.beckmancoulter.com/.../applications/.../ceq_aflp_app_stat_dcr.asp...




Amplified fragment length polymorphism based identification of ...
Amplified fragment length polymorphism based identification of genetic markers and novel PCR assay for differentiation of Campylobacter fetus subspecies ...
jmm.sgmjournals.org/cgi/content/abstract/54/12/1217




Impact of Amplified Fragment Length Polymorphism Size Homoplasy on ...
Impact of Amplified Fragment Length Polymorphism Size Homoplasy on the Estimation of Population Genetic Diversity and the Detection of Selective Loci ...
www.genetics.org/cgi/content/abstract/179/1/539




A Combined Amplified Fragment Length Polymorphism and Randomly ...
(RAPD) and amplified fragment length polymorphisms. and other tree species (Crous 1998). Septoria tritici. (AFLPs) allows a highly efficient generation of ...
www.genetics.org/cgi/reprint/161/4/1497.pdf




Fluorescence-labeled Methylation-sensitive Amplified Fragment ...
Fluorescence-labeled Methylation-sensitive Amplified Fragment Length Polymorphism (FL-MS-AFLP) Analysis for Quantitative Determination of DNA Methylation ...
jjco.oxfordjournals.org/cgi/content/abstract/38/4/317




Amplified fragment length polymorphism analysis of different ...
eral utility of amplified fragment length polymorphisms. (AFLPs) for molecular analysis of insect genomes. For highly. complex genomes, such as those from ...
journals.cambridge.org/.../action/cjoGetFulltext%3Ffulltextid%3D889168...




Amplified Fragment Length Polymorphisms (AFLPs) detected with non ...
anthracis as detected by Amplified fragment length polymorphism markers. J. Bact. 179:818
pubs.nrc-cnrc.gc.ca/ispmb/ispmb15/15255-3.pdf




K-State Research Exchange: Amplified fragment length polymorphism ...
Title: Amplified fragment length polymorphism in Mycosphaerella graminicola. Authors: Kabbage, Mehdi. Graduation Date: May 2007. Type: Dissertation ...
krex.k-state.edu/dspace/handle/2097/255 [cache]




Amplified-Fragment Length Polymorphism Fingerprinting of ...
Amplified-fragment length polymorphism (AFLP) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. ...
jcm.asm.org/cgi/content/abstract/37/10/3300




Using Amplified Fragment Length Polymorphisms and Simple Sequence ...
We have used two DNA-based methods, amplified fragment length polymorphisms (AFLPs) and simple sequence length polymorphisms (SSLPs), to distinguish six ...
crop.scijournals.org/cgi/content/abstract/39/6/1715




Amplified fragment length polymorphisms and sequence data in the ...
4 Phenogram of the neighbor-joining analysis of amplified fragment length polymorphism (AFLP) fingerprints using Nei
www.blackwell-synergy.com/.../pdf/.../j.1469-8137.2007.02172.x




Brazilian Journal of Microbiology - Use of single-enzyme amplified ...
Use of single-enzyme amplified fragment length polymorphism for typing Clostridium ... In the present study amplified fragment length polymorphism analyses ...
www.scielo.br/scielo.php%3Fpid%3DS1517-83822006000300034%26script%3Dsci_art...




BioMed Central Full text Combined use of Amplified Fragment ...
Amplified Fragment Length Polymorphism (AFLP) would scan the entire genome ... Here we evaluate the ability of Amplified Fragment Length Polymorphism (AFLP) ...
www.biomedcentral.com/1471-2334/7/86




Amplified fragment length polymorphism of Streptococcus suis ...
Amplified fragment length polymorphism of Streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background ...
jmm.sgmjournals.org/cgi/content/abstract/56/1/102




Chromosome numbers and pollen stainability of three species of ...
This Article. Right arrow, Full Text. Right arrow, Full Text (PDF). Right arrow, Submit a response. Right arrow, Alert me when this article is cited ...
www.amjbot.org/cgi/content/abstract/88/4/693




Amplified Fragment Length Polymorphism Analysis of Campylobacter ...
The high-resolution genotyping method of amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationships between ...
aem.asm.org/cgi/content/abstract/66/9/3917




Amplified Fragment Length Polymorphism (AFLP) Provides Molecular ...
Amplified fragment length polymorphism (AFLP) analysis using 17 primer combinations was carried out on two species of Caladium (C. bicolor and C. ...
aob.oxfordjournals.org/cgi/content/abstract/84/2/155


Springer Protocols: Abstract: Amplified Fragment Length ...
Amplified Fragment Length Polymorphism Analysis of Salmonella enterica ... Amplified fragment length polymorphism (AFLP) is a powerful PCR-based ...
www.springerprotocols.com/.../doi/.../978-1-59745-512-1_8