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Thursday, February 23, 2012

Purifying DNA from TAE agarose gels

1. Excise band from agarose gel.

2. Add 3 volumes of NaI and incubate at 55°C to melt gel.

3. Add 5μl GLASSMILK suspension.

4. Pellet GLASSMILK/DNA complex (5 seconds).

5. Wash pellet with 1ml NEW Wash.

6. Pellet again and rewash with 0.5ml NEW Wash.

7. Elute DNA with 10-20μl water or 0.1x TE.

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