Animal Perfusion Solution

4% Paraformaldehyde (For ICC & 5' Nucleutidase staining)

100 ml 250 ml 300 ml 500 ml 1000ml

Paraformaldehyde 4g 10g 12g 20g 40g

ddH₂O 80ml 200ml 240ml 400ml 800ml

heat mixture (do not exceed 65 C) with stirring

add a few drops 50% NaOH to clear solution, add:

NaCl 0.8g 2g 2.4g 4g 8g

KCl 0.02g 0.05g 0.06g 0.1g 0.2g

Na₂HPO₄ 0.061g 0.152g 0.182g 0.305g 0.61

KH₂PO₄ 0.02g 0.05g 0.06g 0.1g 0.2g

pH solution to 7.0-7.5 with paper pH strips

Volume to 100ml 250ml 300ml 500ml 1000ml

Filter entire solution

Cool on ice

2% Paraformaldehyde (200 ml)

Paraformaldehyde 4g

ddH₂O 160ml

heat mixture (do not exceed 65 C) with stirring

add a few drops 50% NaOH to clear solution, add:

NaCl 1.6 g

KCl 0.04 g

Na₂HPO₄ 0.122 g

KH₂PO₄ 0.04 g

pH solution to 7.0-7.5 with paper pH strips

Volume to 200 ml

Filter entire solution

Cool on ice