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Sunday, December 21, 2008

Arabidopsis Protocols

Arabidopsis cell suspension and root liquid culture

Arabidopsis EMS mutagenesis of seeds

Arabidopsis genomic DNA prep-CTAB extraction method

Arabidopsis genomic DNA prep-Proteinase K method

Arabidopsis GUS staining

Arabidopsis liquid culture

Arabidopsis mutants

Arabidopsis quick genomic DNA prep for PCR

Arabidopsis root culture

Arabidopsis seed sterilization

Arabidopsis vacuum infiltration (plant transformation)

Confocal Microscopy- Nuclear Staining

Cross-fertilization of Arabidopsis

Crossing Arabidopsis plants (flower emmansculation and flower preparation for fertilization)

Glucocorticoid (GVG)-inducible promoter system for Arabidopsis

How we grow Arabidopsis

In situ hybridization using radioactive probes

Non radioactive in situ hybridization

Plastic Sectioning- Tissue fixation

Protoplast preparation (plant tissue)

Scanning Electron Microscope- Tissue fixation and dissection

Transmission Electron Microscopy- Tissue fixation

Vacuum infiltration of Arabidopsis plants

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