Chapter 9. Cellular Biology

Chapter 9. Cellular Biology

General cell culture protocols

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Introduction

What are the unique properties of all stem cells?

What are embryonic stem cells?

What are adult stem cells?

What are the similarities and differences between embryonic and adult stem cells?

What are the potential uses of human stem cells and the obstacles that must be overcome before these potential uses will be realized?

Where can I get more information?

International Society for Stem Cell Research (ISSCR)

Culture of Human Embryonic Stem Cells (hESC)

Establishment and characterization of human embryonic stem cell line

Rat stem cell isolation and culture

Mouse stem cell isolation and culture

Induction of Stem Cell Differentiation

Cancer stem cells

Video Data on Stem Cell Research (click to show)

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Blood cell fractionation (white blood cell isolation)

Endothelial cell isolation and culture protocols

Flow cytometry (FCM)