SOLUTIONS 1.
Solutions 2.
Sunday, December 21, 2008
Online Resources and Reference Database-1
List A (From: FEROLab)
Journal Search via
Entrez-PubMed at NCBI
Community of Science Database
FHCRC Stockroom (FHCRC only)
Methods & Reagents BIOSCI newsgroup
Technical Tips at BioMedNet
Primer3+ Nucleic Acid Databases
Protein Data Bank at Brookhaven
BioMagResBank Pairwise Alignment Servers
BLAST 2 Sequences against each other
BLOCKS at FHCRC
ClustalW at EMBnet
BOXSHADE for easy viewing
Michigan Tech (Map)
UC Santa Cruz (SAM)
Saturday, October 4, 2008
Bank of Protocols
Protocols for DNA
1 Methylene Blue DNA staining protocol
2 Plasmid Protocols--(31-37)
3 Plasmid Protocols--(21-30)
4 Plasmid Protocol--(1-10)
5 Microsatellites Protocols
6 In-Situ hybridization to Embryonic Tissue Section
7 DNA ligation Protocol
8 Colony Hybridization Protocols
9 Colony Hybridization
10 DNA Isolation, Purification and Troubleshooting
11 Deoxyribose Isolation from DNA Degrasion
12 Mitochondrial DNA Isolation
13 Genomic DNA Isolation from Specific Samples
14 Genome DNA Extraction
Protocols for RNA
1 IN VITRO TRANSCRIPTION OF RNA
2 SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)
3 Protocols for SiRNA
4 SELEX—in vitro selection
5 Nucleic Acids Research Methods (2)
6 RNA MicroArray Protocol
7 RNA Isolation
Protocols for PCR
1 PCR Based Molecular Cloning
2 Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
3 Introduction to PCR
4 General PCR introduction
5 PCR Animations
6 PCR, RT PCR and Real Time PCR Tutorials
7 PCR Application Manual
8 PCR Elisa
9 PCR RFLP
10 PCR SSCP
11 Real Time PCR
12 RT-PCR Protocols
13 Variants of PCR (2)
14 Variants of PCR (1)
15 AFLP PCR
16 Alu-PCR
17 Asymmetric PCR
18 Colony PCR Protocols
19 Competitive and Quantitative RT-PCR
20 Degenerate PCR
21 Differential Display PCR
22 In Situ PCR
23 Inverse PCR
24 Ligation Mediated Suppression PCR
25 Long PCR Protocols
26 Methylation Specific PCR
27 Multiplex PCR
28 Nested PCR
29 RACE PCR
30 RAPD PCR
31 Rep-PCR
32 TAIL PCR
33 Touchdown PCR
34 Vectorette PCR
Protocols for Protein
1 Enzyme Assay（43-47）
2 Enzyme Assay（36-42）
3 Enzyme Assay（22-28）
4 Protocols for In Vitro Translation
5 Dot Blot Protocols
6 Selected Protocols of Protein Purification
7 Western Blotting Protocols (1)
Protocols for Cells
1 FACS for Bone marrow macrophages
2 Bone Marrow Macrophages
3 Metabolic Labeling of Cells with 35S
4 Protocols from the science advisory board
5 Introduction to Animal Cell Culture
6 Cell Transfection Protocols
7 Laser Capture Microdissection Protocols
8 Stem Cell Research Techniques and Protocols
9 XTT Cell Viability Assay Protocol
10 Apoptosis Assay Protocols
Protocols for Genetics
1 Genetic Analyses for DNA Protein Interactions
2 Comet Assay
3 Epigenetics Protocols
Protocols for Immunology and IHC
1 Grocott's Methenamine Silver (GMS) stain and troubleshooting
2 Elegans Immunohistochemistry
3 Cytokine Assay Protocols
4 Fluorescence in situ hybridization (FISH)
5 Radioimmunoassay--RIA Protocols
6 Competitive ELISA Protocols
7 Immunohistochemistry Stain for Frozen tissue
Protocols for Chromatography
1 Polysaccharide sequencing
2 Application Guides and Protocols (Nest Group)
3 HPLC Troubleshooting
4 Introduction to HPLC
Protocols for Model Organisms
1 Yeast Protocols
Protocols for Animal Experiment
1 Murine Models of Human Breast Cancer (B)
2 Chorioallantoic Membrane Vascular Assay
3 Guidelines for Techniques with Rodents (Duke University)
4 Tail Vein Injection Techniques
5 Animal Search Engines
Protocols for Basic Techniques
1 H & E Stain Troubleshooting
2 H and E Stain Protocols
3 Electrophoresis Protocols (11-26)
4 Lab. Safety in Life Science
1 Methylene Blue DNA staining protocol
2 Plasmid Protocols--(31-37)
3 Plasmid Protocols--(21-30)
4 Plasmid Protocol--(1-10)
5 Microsatellites Protocols
6 In-Situ hybridization to Embryonic Tissue Section
7 DNA ligation Protocol
8 Colony Hybridization Protocols
9 Colony Hybridization
10 DNA Isolation, Purification and Troubleshooting
11 Deoxyribose Isolation from DNA Degrasion
12 Mitochondrial DNA Isolation
13 Genomic DNA Isolation from Specific Samples
14 Genome DNA Extraction
Protocols for RNA
1 IN VITRO TRANSCRIPTION OF RNA
2 SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)
3 Protocols for SiRNA
4 SELEX—in vitro selection
5 Nucleic Acids Research Methods (2)
6 RNA MicroArray Protocol
7 RNA Isolation
Protocols for PCR
1 PCR Based Molecular Cloning
2 Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
3 Introduction to PCR
4 General PCR introduction
5 PCR Animations
6 PCR, RT PCR and Real Time PCR Tutorials
7 PCR Application Manual
8 PCR Elisa
9 PCR RFLP
10 PCR SSCP
11 Real Time PCR
12 RT-PCR Protocols
13 Variants of PCR (2)
14 Variants of PCR (1)
15 AFLP PCR
16 Alu-PCR
17 Asymmetric PCR
18 Colony PCR Protocols
19 Competitive and Quantitative RT-PCR
20 Degenerate PCR
21 Differential Display PCR
22 In Situ PCR
23 Inverse PCR
24 Ligation Mediated Suppression PCR
25 Long PCR Protocols
26 Methylation Specific PCR
27 Multiplex PCR
28 Nested PCR
29 RACE PCR
30 RAPD PCR
31 Rep-PCR
32 TAIL PCR
33 Touchdown PCR
34 Vectorette PCR
Protocols for Protein
1 Enzyme Assay（43-47）
2 Enzyme Assay（36-42）
3 Enzyme Assay（22-28）
4 Protocols for In Vitro Translation
5 Dot Blot Protocols
6 Selected Protocols of Protein Purification
7 Western Blotting Protocols (1)
Protocols for Cells
1 FACS for Bone marrow macrophages
2 Bone Marrow Macrophages
3 Metabolic Labeling of Cells with 35S
4 Protocols from the science advisory board
5 Introduction to Animal Cell Culture
6 Cell Transfection Protocols
7 Laser Capture Microdissection Protocols
8 Stem Cell Research Techniques and Protocols
9 XTT Cell Viability Assay Protocol
10 Apoptosis Assay Protocols
Protocols for Genetics
1 Genetic Analyses for DNA Protein Interactions
2 Comet Assay
3 Epigenetics Protocols
Protocols for Immunology and IHC
1 Grocott's Methenamine Silver (GMS) stain and troubleshooting
2 Elegans Immunohistochemistry
3 Cytokine Assay Protocols
4 Fluorescence in situ hybridization (FISH)
5 Radioimmunoassay--RIA Protocols
6 Competitive ELISA Protocols
7 Immunohistochemistry Stain for Frozen tissue
Protocols for Chromatography
1 Polysaccharide sequencing
2 Application Guides and Protocols (Nest Group)
3 HPLC Troubleshooting
4 Introduction to HPLC
Protocols for Model Organisms
1 Yeast Protocols
Protocols for Animal Experiment
1 Murine Models of Human Breast Cancer (B)
2 Chorioallantoic Membrane Vascular Assay
3 Guidelines for Techniques with Rodents (Duke University)
4 Tail Vein Injection Techniques
5 Animal Search Engines
Protocols for Basic Techniques
1 H & E Stain Troubleshooting
2 H and E Stain Protocols
3 Electrophoresis Protocols (11-26)
4 Lab. Safety in Life Science
Bank of Bioprotocols
1.General Lab Techniques
2. Lab security and basic techniques
3. Advanced lab skills-(1)
4. Advanced lab skills-(2)
5. Molecular Biology
6. DNA isolation & related protocols
7. DNA Purification (glass milk vs electroelution)
8. DNA and RNA sequencing
9. Nucleic acid methods (1)
10.Nucleic acid methods (2)
11.Isolation of DNA,RNA, and Protein simultaneously.
12.DNA mutation detection by SSCP
13.Mouse genotyping by PCR
14.PCR,RT-PCR,Real time PCR etc.
15.Southern blot hybridization
16. Loss of Heterozygosity (LOH)
17.Gene knockout protocol
18.RNA Isolation and Purification
19.Preparation of DNA and RNA probes
20.Northern blot hybridization
21.SiRNA gene knockout
22.Western blot hybridization
23.Molecular cloning
24.Conditional gene transfection(Tet on/off)
25.Protein sequencing
26.Protein labeling techniques
27.Subcellular fractionations
28.EMSA
29.Methylation interference
30.Southwestern blotting
31.Filter binding protocols
32.Footpring protocols
33.DNA (RNA) protein interactions
34.Plasmid and its usefulness
35.DNA library construction
36.Microarray protocols.
37. Protein chips
38.Detecting of protein phosphorylation
39.Protein methods
40.Molecular separation
41.Gene therapy for cancer
42.X-ray crystallography
43.Metabolism and chromatographies
44.GC/MS Background
45.Glucose metabolism and its related protocols
46.PAS staining
47.Polysaccharide sequencing
48.Deoxyribose procedure
49.Ribose metabolism analysis
50.Lactate cleanup and derivative
51.Amino acids
52.Fatty acids
53.Lipid protocols
54.Cholesterol
55.Bile acids
56.Urea procedure
57.Choline Incorporation Assay
58.Isotope Ratio Mass Spectrometer (IRMS)
59.Liquid chromatography / mass spectrometer (LC/MS)
60.Proteomics
61.NMR protocols and tutorials
62.HPLC protocols
63.TLC
64.Molecular labeling
65.Immunology/immunohistochemistry
66.Preparing silanized (+plus) slides
67.Histological fixation
68.Microwave histology
69.Microscopy Techniques
70. Electron microscopy
71.HE staining
72.Nucleic acid stain
73.Special cell & cell fraction stains
74.Antibody purification
75.Antibody storage and handling
76.Conjugation of monoclonal antibodies
77.Antigen retrieval
78.Elisa
79.FRQs for histochemistry
80.Immunoperoxidase staining techniques
81.Immunofluorohistochemistry
82.Confocal microscope technique
83.Laser Capture Microdissection
84.Immunoprecipitation
85.ChIP assay
86.Hybridization in situ
87.Histotechnology--technical methods
88. Flow cytometry (FCM)
89.Kinase assay
90.Cellular Biology
91.General cell culture protocols
92.Chromosome karyotype
93.Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
94.Cell cycle assay
95.Migration assay
96.Stem cell & related protocols
97.Apoptosis and related protocols
98.Soft Agar Assay for Colony Formation
99.Aorta ring assay
100.GFP transfection
101.Gene transfection
102.Transformation protocols
103.Blood cell fractionation (white blood cell isolation)
104.Endothelial isolation and culture
105.Isolation and culture human brain microvessel endothelial cells
106.Animal Experiments
107.Blood sampling from animals
108.Basic skills for animal experiments
109.Cancer xenograft animal models
110.Transgenic animal procedures
111.Transgenic cancer models
112.Animal search
113.Animal models for depression-like and anxiety-like behavior
114.Genetics
115.Epigenetics protocols
116.SNPs
117.Genetic analysis
118.Mutagenesis
119.Model Organisms
120.C. Elegans
121.Yeast protocols and resource
2. Lab security and basic techniques
3. Advanced lab skills-(1)
4. Advanced lab skills-(2)
5. Molecular Biology
6. DNA isolation & related protocols
7. DNA Purification (glass milk vs electroelution)
8. DNA and RNA sequencing
9. Nucleic acid methods (1)
10.Nucleic acid methods (2)
11.Isolation of DNA,RNA, and Protein simultaneously.
12.DNA mutation detection by SSCP
13.Mouse genotyping by PCR
14.PCR,RT-PCR,Real time PCR etc.
15.Southern blot hybridization
16. Loss of Heterozygosity (LOH)
17.Gene knockout protocol
18.RNA Isolation and Purification
19.Preparation of DNA and RNA probes
20.Northern blot hybridization
21.SiRNA gene knockout
22.Western blot hybridization
23.Molecular cloning
24.Conditional gene transfection(Tet on/off)
25.Protein sequencing
26.Protein labeling techniques
27.Subcellular fractionations
28.EMSA
29.Methylation interference
30.Southwestern blotting
31.Filter binding protocols
32.Footpring protocols
33.DNA (RNA) protein interactions
34.Plasmid and its usefulness
35.DNA library construction
36.Microarray protocols.
37. Protein chips
38.Detecting of protein phosphorylation
39.Protein methods
40.Molecular separation
41.Gene therapy for cancer
42.X-ray crystallography
43.Metabolism and chromatographies
44.GC/MS Background
45.Glucose metabolism and its related protocols
46.PAS staining
47.Polysaccharide sequencing
48.Deoxyribose procedure
49.Ribose metabolism analysis
50.Lactate cleanup and derivative
51.Amino acids
52.Fatty acids
53.Lipid protocols
54.Cholesterol
55.Bile acids
56.Urea procedure
57.Choline Incorporation Assay
58.Isotope Ratio Mass Spectrometer (IRMS)
59.Liquid chromatography / mass spectrometer (LC/MS)
60.Proteomics
61.NMR protocols and tutorials
62.HPLC protocols
63.TLC
64.Molecular labeling
65.Immunology/immunohistochemistry
66.Preparing silanized (+plus) slides
67.Histological fixation
68.Microwave histology
69.Microscopy Techniques
70. Electron microscopy
71.HE staining
72.Nucleic acid stain
73.Special cell & cell fraction stains
74.Antibody purification
75.Antibody storage and handling
76.Conjugation of monoclonal antibodies
77.Antigen retrieval
78.Elisa
79.FRQs for histochemistry
80.Immunoperoxidase staining techniques
81.Immunofluorohistochemistry
82.Confocal microscope technique
83.Laser Capture Microdissection
84.Immunoprecipitation
85.ChIP assay
86.Hybridization in situ
87.Histotechnology--technical methods
88. Flow cytometry (FCM)
89.Kinase assay
90.Cellular Biology
91.General cell culture protocols
92.Chromosome karyotype
93.Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
94.Cell cycle assay
95.Migration assay
96.Stem cell & related protocols
97.Apoptosis and related protocols
98.Soft Agar Assay for Colony Formation
99.Aorta ring assay
100.GFP transfection
101.Gene transfection
102.Transformation protocols
103.Blood cell fractionation (white blood cell isolation)
104.Endothelial isolation and culture
105.Isolation and culture human brain microvessel endothelial cells
106.Animal Experiments
107.Blood sampling from animals
108.Basic skills for animal experiments
109.Cancer xenograft animal models
110.Transgenic animal procedures
111.Transgenic cancer models
112.Animal search
113.Animal models for depression-like and anxiety-like behavior
114.Genetics
115.Epigenetics protocols
116.SNPs
117.Genetic analysis
118.Mutagenesis
119.Model Organisms
120.C. Elegans
121.Yeast protocols and resource
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