Bank of Bioprotocols

1.General Lab Techniques
2. Lab security and basic techniques
3. Advanced lab skills-(1)
4. Advanced lab skills-(2)
5. Molecular Biology
6. DNA isolation & related protocols
7. DNA Purification (glass milk vs electroelution)
8. DNA and RNA sequencing
9. Nucleic acid methods (1)
10.Nucleic acid methods (2)
11.Isolation of DNA,RNA, and Protein simultaneously.
12.DNA mutation detection by SSCP
13.Mouse genotyping by PCR
14.PCR,RT-PCR,Real time PCR etc.
15.Southern blot hybridization
16. Loss of Heterozygosity (LOH)
17.Gene knockout protocol
18.RNA Isolation and Purification
19.Preparation of DNA and RNA probes
20.Northern blot hybridization
21.SiRNA gene knockout
22.Western blot hybridization
23.Molecular cloning
24.Conditional gene transfection(Tet on/off)
25.Protein sequencing
26.Protein labeling techniques
27.Subcellular fractionations
28.EMSA
29.Methylation interference
30.Southwestern blotting
31.Filter binding protocols
32.Footpring protocols
33.DNA (RNA) protein interactions
34.Plasmid and its usefulness
35.DNA library construction
36.Microarray protocols.
37. Protein chips
38.Detecting of protein phosphorylation
39.Protein methods
40.Molecular separation
41.Gene therapy for cancer
42.X-ray crystallography
43.Metabolism and chromatographies
44.GC/MS Background
45.Glucose metabolism and its related protocols
46.PAS staining
47.Polysaccharide sequencing
48.Deoxyribose procedure
49.Ribose metabolism analysis
50.Lactate cleanup and derivative
51.Amino acids
52.Fatty acids
53.Lipid protocols
54.Cholesterol
55.Bile acids
56.Urea procedure
57.Choline Incorporation Assay
58.Isotope Ratio Mass Spectrometer (IRMS)
59.Liquid chromatography / mass spectrometer (LC/MS)
60.Proteomics
61.NMR protocols and tutorials
62.HPLC protocols
63.TLC
64.Molecular labeling
65.Immunology/immunohistochemistry
66.Preparing silanized (+plus) slides
67.Histological fixation
68.Microwave histology
69.Microscopy Techniques
70. Electron microscopy
71.HE staining
72.Nucleic acid stain
73.Special cell & cell fraction stains
74.Antibody purification
75.Antibody storage and handling
76.Conjugation of monoclonal antibodies
77.Antigen retrieval
78.Elisa
79.FRQs for histochemistry
80.Immunoperoxidase staining techniques
81.Immunofluorohistochemistry
82.Confocal microscope technique
83.Laser Capture Microdissection
84.Immunoprecipitation
85.ChIP assay
86.Hybridization in situ
87.Histotechnology--technical methods
88. Flow cytometry (FCM)
89.Kinase assay
90.Cellular Biology
91.General cell culture protocols
92.Chromosome karyotype
93.Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
94.Cell cycle assay
95.Migration assay
96.Stem cell & related protocols
97.Apoptosis and related protocols
98.Soft Agar Assay for Colony Formation
99.Aorta ring assay
100.GFP transfection
101.Gene transfection
102.Transformation protocols
103.Blood cell fractionation (white blood cell isolation)
104.Endothelial isolation and culture
105.Isolation and culture human brain microvessel endothelial cells
106.Animal Experiments
107.Blood sampling from animals
108.Basic skills for animal experiments
109.Cancer xenograft animal models
110.Transgenic animal procedures
111.Transgenic cancer models
112.Animal search
113.Animal models for depression-like and anxiety-like behavior
114.Genetics
115.Epigenetics protocols
116.SNPs
117.Genetic analysis
118.Mutagenesis
119.Model Organisms
120.C. Elegans
121.Yeast protocols and resource