The Rep-PCR Genomic Fingerprinting Home PageMichigan State University
E. coli Source Tracking by Rep-PCR DNA FingerprintingThe Regents of the University of Minnesota
How does Rep-PCR workThe Rep-PCR Genomic Fingerprinting Home Page
Genomic Fingerprinting by Amplification of Rep-PCR.
Springer Protocols
Rep-PCR DNA Fingerprinting and Computer Analysis (University of Minnesota)
Overview (10-25-00)
Box Fingerprint Protocol (3-4-02)
Running and Staining Box Fingerprint Gels (10-25-00)
REP-PCR Method Overview (University of Minnesota)
Rep-PCR fingerprinting protocol (University of Minnesota)
https://www.msu.edu/~debruijn/dna5-11.htm
Wednesday, September 10, 2008
Tail PCR
An introduction of TAIL PCR
Many product bands from the primary TAIL-PCR reaction disappeared after the secondary TAIL-PCR, indicating that these were non-specific type II products ...ihome.cuhk.edu.hk/~z045478/TAIL-PCR%5Bfinal%5D.ppt
TAIL-PCR to clone flanking sequence from Feldmann's linesChun-Ming Liu's Homepage
TAIL PCR ANALYSIS
TAIL PCR ANALYSIS. For this service Investigators have three purification options. Our standard purification protocol from the Jackson laboratory, ...www.lajollaneuroscience.org/sr/homepage/dna/tailpcranalysis.html
TAIL PCR ProtocolThe Preuss LabDivision of Biological Sciences, University of Chicago
TAIL PCR
Below is a summary of the responses: I've been using TAIL PCR to isolate the ends of BACs during my chromosome walk. I obtained the sequences of my ...net.bio.net/bionet/mm/arab-gen/1998-September/006602.html
Tail PCR Protocol
Purpose: Mu TAIL PCR produces a population of fragments with one end anchored in Robertson's Mutator (Mu) TIR's and the other in the Mu flanking DNA. ...currant.hos.ufl.edu/mutail/tail.htm
Springer Protocols:High-Throughput TAIL-PCR as a tool to identify DNA flanking insertions.
Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.www.springerprotocols.com/Abstract/doi/10.1385/1-59259-413-1:241
TAIL-PCR to clone flanking sequence from Feldmann's lines
The first thing you need to do before starting your TAIL-PCR is to figure out which border of the T-DNA is flanking the plant genome, especially with Ken ...http://www.plant.wageningen-ur.nl/projects/hybtech/Liu/tail_PCR.htm
Isolation of genomic fragment by TAIL-PCR
More than 50bp must be remained downstream of a third gene specific primer to check if a gained TAIL PCR fragment is correctly extended from an original ...www.nibb.ac.jp/evodevo/PHYSCOmanual/4.3.htm
Many product bands from the primary TAIL-PCR reaction disappeared after the secondary TAIL-PCR, indicating that these were non-specific type II products ...ihome.cuhk.edu.hk/~z045478/TAIL-PCR%5Bfinal%5D.ppt
TAIL-PCR to clone flanking sequence from Feldmann's linesChun-Ming Liu's Homepage
TAIL PCR ANALYSIS
TAIL PCR ANALYSIS. For this service Investigators have three purification options. Our standard purification protocol from the Jackson laboratory, ...www.lajollaneuroscience.org/sr/homepage/dna/tailpcranalysis.html
TAIL PCR ProtocolThe Preuss LabDivision of Biological Sciences, University of Chicago
TAIL PCR
Below is a summary of the responses: I've been using TAIL PCR to isolate the ends of BACs during my chromosome walk. I obtained the sequences of my ...net.bio.net/bionet/mm/arab-gen/1998-September/006602.html
Tail PCR Protocol
Purpose: Mu TAIL PCR produces a population of fragments with one end anchored in Robertson's Mutator (Mu) TIR's and the other in the Mu flanking DNA. ...currant.hos.ufl.edu/mutail/tail.htm
Springer Protocols:High-Throughput TAIL-PCR as a tool to identify DNA flanking insertions.
Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.www.springerprotocols.com/Abstract/doi/10.1385/1-59259-413-1:241
TAIL-PCR to clone flanking sequence from Feldmann's lines
The first thing you need to do before starting your TAIL-PCR is to figure out which border of the T-DNA is flanking the plant genome, especially with Ken ...http://www.plant.wageningen-ur.nl/projects/hybtech/Liu/tail_PCR.htm
Isolation of genomic fragment by TAIL-PCR
More than 50bp must be remained downstream of a third gene specific primer to check if a gained TAIL PCR fragment is correctly extended from an original ...www.nibb.ac.jp/evodevo/PHYSCOmanual/4.3.htm
Touchdown PCR
Background: Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers will avoid amplifying nonspecific sequence. The temperature at which primers anneal during a cycle of polymerase chain reaction determines the specificity of annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just below this point, only very specific base pairing between the primer and the template will occur. At lower temperatures, the primers bind less specifically. Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in early steps of amplification will "swamp out" any specific sequences because of the exponential nature of polymerase amplification.
The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures. The annealing temperature is decreased in increments for every subsequent set of cycles (the number of individual cycles and increments of temperature decrease is chosen by the experimenter). The primer will anneal at the highest temperature which is least-permissive of nonspecific binding that it is able to tolerate. Thus, the first sequence amplified is the one between the regions of greatest primer specificity; it is most likely that this is the sequence of interest. These fragments will be further amplified during subsequent rounds at lower temperatures, and will out compete the nonspecific sequences to which the primers may bind at those lower temperatures. If the primer initially binds to the sequence of interest at a low temperature, subsequent rounds of polymerase chain reaction can be performed upon the product to further amplify those fragments.
References
Don R, Cox P, Wainwright B, Baker K, Mattick J (1991). "'Touchdown' PCR to circumvent spurious priming during gene amplification". Nucleic Acids Res 19 (14): 4008. doi:10.1093/nar/19.14.4008. PMID 1861999.
Roux K (1994). "Using mismatched primer-template pairs in touchdown PCR". Biotechniques 16 (5): 812-4. PMID 8068332.
Hecker K, Roux K (1996). "High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR". Biotechniques 20 (3): 478-85. PMID 8679209
Berglund:Touchdown PCROpenWetWare
'Touchdown' PCR to circumvent spurious priming during gene amplificationDon RH et al. Nucleic Acids Res. 1991 Jul 25;19(14):4008.
TouchDown PCR
Bio.net
Protocols for PCR and Touchdown PCR:
www.jcsg.org/prod/scripts/primer/pcr_protocol.doc
Combining Multiplex and touchdown PCR for Microsatellite Analysis.
Springer Protocols
Touchdown' PCR to circumvent spurious priming during gene amplification.
Nucleic Acid Res
The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures. The annealing temperature is decreased in increments for every subsequent set of cycles (the number of individual cycles and increments of temperature decrease is chosen by the experimenter). The primer will anneal at the highest temperature which is least-permissive of nonspecific binding that it is able to tolerate. Thus, the first sequence amplified is the one between the regions of greatest primer specificity; it is most likely that this is the sequence of interest. These fragments will be further amplified during subsequent rounds at lower temperatures, and will out compete the nonspecific sequences to which the primers may bind at those lower temperatures. If the primer initially binds to the sequence of interest at a low temperature, subsequent rounds of polymerase chain reaction can be performed upon the product to further amplify those fragments.
References
Don R, Cox P, Wainwright B, Baker K, Mattick J (1991). "'Touchdown' PCR to circumvent spurious priming during gene amplification". Nucleic Acids Res 19 (14): 4008. doi:10.1093/nar/19.14.4008. PMID 1861999.
Roux K (1994). "Using mismatched primer-template pairs in touchdown PCR". Biotechniques 16 (5): 812-4. PMID 8068332.
Hecker K, Roux K (1996). "High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR". Biotechniques 20 (3): 478-85. PMID 8679209
Berglund:Touchdown PCROpenWetWare
'Touchdown' PCR to circumvent spurious priming during gene amplificationDon RH et al. Nucleic Acids Res. 1991 Jul 25;19(14):4008.
TouchDown PCR
Bio.net
Protocols for PCR and Touchdown PCR:
www.jcsg.org/prod/scripts/primer/pcr_protocol.doc
Combining Multiplex and touchdown PCR for Microsatellite Analysis.
Springer Protocols
Touchdown' PCR to circumvent spurious priming during gene amplification.
Nucleic Acid Res
Vectorette PCR
Vectorette End Rescue of Bacterial ClonesStéphanie Le Hellard, University of Edinburgh
Vectorette PCR of mTn-3xHA
The PCR product may then be isolated and sequenced. The vectorette PCR method has the advantage of eliminating transformation and cloning steps. ...ygac.med.yale.edu/mtn/reagent/avail_reagents/vectorette_PCR.html
Vectorette PCR of Yeast DNA
Vectorette PCR of Yeast DNA. Carl Friddle. 1) Cut 1-3 µg of clean DNA overnight with 8-10U of blunt cutting enzyme in 20µl. Most problems come from dirty, ...genomics.princeton.edu/botstein/protocols/vectorette.html
Vectorette PCR Methods
Vectorette PCR was designed for amplification of DNA regions adjacent to known regions ... Performing vectorette PCR with multiple vectorette libraries can ...http://www.bio.psu.edu/People/Faculty/Akashi/vectPCR.html
A Vectorette PCR-based approach for sequencing homologous regions from different genomes.For a successful vectorette PCR, a good specific PCR primer is critical. .... sequencing confirmed that vectorette PCR successfully amplified all expected ...http://www.bio.psu.edu/People/Faculty/Akashi/vect_PCR.online.072304.pdf
Vectorette PCR: a novel approach to genomic walking.
PCR Methods Appl. C Arnold and I J Hodgson. Vectorette PCR: a novel approach to genomic ... genetics method, or vectorette PCR, are. shown in Figure 1. ...www.genome.org/cgi/reprint/1/1/39.pdf
Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers
We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR ...http://nar.oxfordjournals.org/cgi/content/full/24/11/2190
Springer Protocols:Long Distance Vectorette PCR (LDV PCR).
Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.www.springerprotocols.com/Abstract/doi/10.1385/1-59259-177-9:275
Vectorette PCR of mTn-3xHA
The PCR product may then be isolated and sequenced. The vectorette PCR method has the advantage of eliminating transformation and cloning steps. ...ygac.med.yale.edu/mtn/reagent/avail_reagents/vectorette_PCR.html
Vectorette PCR of Yeast DNA
Vectorette PCR of Yeast DNA. Carl Friddle. 1) Cut 1-3 µg of clean DNA overnight with 8-10U of blunt cutting enzyme in 20µl. Most problems come from dirty, ...genomics.princeton.edu/botstein/protocols/vectorette.html
Vectorette PCR Methods
Vectorette PCR was designed for amplification of DNA regions adjacent to known regions ... Performing vectorette PCR with multiple vectorette libraries can ...http://www.bio.psu.edu/People/Faculty/Akashi/vectPCR.html
A Vectorette PCR-based approach for sequencing homologous regions from different genomes.For a successful vectorette PCR, a good specific PCR primer is critical. .... sequencing confirmed that vectorette PCR successfully amplified all expected ...http://www.bio.psu.edu/People/Faculty/Akashi/vect_PCR.online.072304.pdf
Vectorette PCR: a novel approach to genomic walking.
PCR Methods Appl. C Arnold and I J Hodgson. Vectorette PCR: a novel approach to genomic ... genetics method, or vectorette PCR, are. shown in Figure 1. ...www.genome.org/cgi/reprint/1/1/39.pdf
Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers
We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR ...http://nar.oxfordjournals.org/cgi/content/full/24/11/2190
Springer Protocols:Long Distance Vectorette PCR (LDV PCR).
Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.www.springerprotocols.com/Abstract/doi/10.1385/1-59259-177-9:275
Web Source of PCR (3)
Polymerase Chain Reaction (PCR) A graphic description of the principle of PCR from the US National Health Museum web site.http://www.accessexcellence.org/AB/GG/polymerase.html
Principle of PCR Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/principles/pcr.html
Protocol for T-RFLP by Capillary Electrophoresis Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grntzig, B Stres, HL Ayala del Ro and JM Tiedje, Center for Microbial Ecology, Michigan State University, USAhttp://rdp.cme.msu.edu/html/t-rflp_jul02.html
Protocols Online: PCR Protocols Extensive collection of PCR protocols and methods from Protocol On Line.http://www.protocol-online.org/prot/Molecular_Biology/PCR/
Quantitative PCR Protocol From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/cyto/quanpcr.html
RACE amplification Rapid Amplification of cDNA Ends. Protocol, from National Institute of Genetics, Japan.http://www.nig.ac.jp/labs/EarlyEmb/midline/protocol/RACE.html
RAPD PCR RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.http://avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
RAPD analysis with P. infestans Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.http://www.fgsc.net/methods/Judel.html
RAPD's Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html
RFLP Definition RFLP = Restriction Fragment Length Polymorphism, from FDAhttp://vm.cfsan.fda.gov/~frf/rflp.html
Random Arbitrarily Primed PCR Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.http://www.enterococcus.ouhsc.edu/random_pcr.asp
Rational primer design greatly improves differential display-PCR (DD-PCR) Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.http://nar.oupjournals.org/cgi/content/full/25/11/2239
Reference in PCR Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.http://www.gene-quantification.org/
Rep-PCR Genomic Fingerprinting Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.http://www.msu.edu/user/debruijn/
Roe Laboratory Protocols Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.http://www.genome.ou.edu/proto.html
Single tube confirmation PCR protocol For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html
Standard PCR protocols From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.http://web.uct.ac.za/microbiology/pcrcond.htm
T-DNA Generated Enhancer Traps in Arabidopsis Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.http://www.dartmouth.edu/~tjack/
Tavi's PCR protocols A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Technical manual of PCR Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.http://web.uct.ac.za/microbiology/pcr.htm
The Polymerase Chain Reaction A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology.http://www.faseb.org/opar/bloodsupply/pcr.html
The web guide of PCR List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.http://www.pcrlinks.com/
Thermostable DNA Polymerases Discussion of their origin and briefly their properties. From the web site of Colorado State University.http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html
Wayward PCR primers Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html
What the Heck is PCR? Popular description of the PCR technique by John C Brown, University of Kansas 1995.http://people.ku.edu/~jbrown/pcr.html
Which DNA Marker for Which Purpose? Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Gttingen.http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker
quot; Technique for Enhancing Detection of Small Deletions in Mutant Libraries This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/poison1.html
Principle of PCR Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/principles/pcr.html
Protocol for T-RFLP by Capillary Electrophoresis Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grntzig, B Stres, HL Ayala del Ro and JM Tiedje, Center for Microbial Ecology, Michigan State University, USAhttp://rdp.cme.msu.edu/html/t-rflp_jul02.html
Protocols Online: PCR Protocols Extensive collection of PCR protocols and methods from Protocol On Line.http://www.protocol-online.org/prot/Molecular_Biology/PCR/
Quantitative PCR Protocol From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/cyto/quanpcr.html
RACE amplification Rapid Amplification of cDNA Ends. Protocol, from National Institute of Genetics, Japan.http://www.nig.ac.jp/labs/EarlyEmb/midline/protocol/RACE.html
RAPD PCR RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.http://avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
RAPD analysis with P. infestans Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.http://www.fgsc.net/methods/Judel.html
RAPD's Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html
RFLP Definition RFLP = Restriction Fragment Length Polymorphism, from FDAhttp://vm.cfsan.fda.gov/~frf/rflp.html
Random Arbitrarily Primed PCR Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.http://www.enterococcus.ouhsc.edu/random_pcr.asp
Rational primer design greatly improves differential display-PCR (DD-PCR) Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.http://nar.oupjournals.org/cgi/content/full/25/11/2239
Reference in PCR Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.http://www.gene-quantification.org/
Rep-PCR Genomic Fingerprinting Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.http://www.msu.edu/user/debruijn/
Roe Laboratory Protocols Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.http://www.genome.ou.edu/proto.html
Single tube confirmation PCR protocol For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html
Standard PCR protocols From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.http://web.uct.ac.za/microbiology/pcrcond.htm
T-DNA Generated Enhancer Traps in Arabidopsis Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.http://www.dartmouth.edu/~tjack/
Tavi's PCR protocols A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Technical manual of PCR Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.http://web.uct.ac.za/microbiology/pcr.htm
The Polymerase Chain Reaction A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology.http://www.faseb.org/opar/bloodsupply/pcr.html
The web guide of PCR List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.http://www.pcrlinks.com/
Thermostable DNA Polymerases Discussion of their origin and briefly their properties. From the web site of Colorado State University.http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html
Wayward PCR primers Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html
What the Heck is PCR? Popular description of the PCR technique by John C Brown, University of Kansas 1995.http://people.ku.edu/~jbrown/pcr.html
Which DNA Marker for Which Purpose? Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Gttingen.http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker
quot; Technique for Enhancing Detection of Small Deletions in Mutant Libraries This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/poison1.html
Web Source of PCR (2)
Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.http://www.fhcrc.org/labs/gottschling...
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.http://www.fruitfly.org/about/methods...
Inverse PCR for PAC-end sequencing To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.http://www.genetics.wustl.edu/fish_la...
Inverse PCR protocol step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
Kary B. Mullis - Autobiography The originator of PCR, from the Nobel e-museum web site.http://nobelprize.org/chemistry/laure...
Kary Mullis Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.http://www.invent.org/hall_of_fame/10...
Life at High Temperatures Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.http://www.bact.wisc.edu/Bact303/b27
Long PCR Protocol Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USAhttp://twod.med.harvard.edu/labgc/est...
MJ Research Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.http://www.mjr.com/
Molecular Staging, Inc. Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT.http://www.molecularstaging.com/Pages...
Mutation Detection Systems - SSCP Analysis Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London, UK.http://www.ich.ucl.ac.uk/cmgs/sscp.htm
Nematode ITS1 Size Variation Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.http://nematode.unl.edu/its_id/EXAMPL...
Optimizing Multiplex and LA-PCR with Betaine LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.http://www-lecb.ncifcrf.gov/~pnh/pape...
Optimizing PCR Protocols Brief guidelines. From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/imr/optimize_pcr.html
PCR Amplification of cDNA Segments by 2 Stage Nested PCR Protocol from the method database of NIH, USA.http://www.ncbi.nlm.nih.gov/SNP/snp_v...
PCR Project Presentations from the University of California at Berkley on PCR, both current research reports and reviews.http://sunsite.berkeley.edu/biotech/pcr/
PCR Protocol Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
PCR Protocols Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State Universityhttp://www.cas.psu.edu/docs/CASDEPT/V...
PCR Technology An introduction by Connie Veilleux from the US National Health Museum website.http://www.accessexcellence.org/LC/SS...
PCR Troubleshooting Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.http://info.med.yale.edu/genetics/war...
PCR and multiplex PCR guide Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.http://www.info.med.yale.edu/genetics...
PCR animated Animation illustrating the principle of PCR, from the University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/pr...
PCR method protocols Protocols for PCR posted by the Helen Donis-Keller Laboratory.http://hg.wustl.edu/hdk_lab_manual/pc...
PCR primer design and reaction optimisation Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.http://web.uct.ac.za/microbiology/pcr...
PCR protocols Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School.http://genome.wustl.edu/tools/protoco...
Polymerase Chain Reaction Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.http://www.accessexcellence.com/RC/CT...
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.http://www.fruitfly.org/about/methods...
Inverse PCR for PAC-end sequencing To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.http://www.genetics.wustl.edu/fish_la...
Inverse PCR protocol step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
Kary B. Mullis - Autobiography The originator of PCR, from the Nobel e-museum web site.http://nobelprize.org/chemistry/laure...
Kary Mullis Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.http://www.invent.org/hall_of_fame/10...
Life at High Temperatures Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.http://www.bact.wisc.edu/Bact303/b27
Long PCR Protocol Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USAhttp://twod.med.harvard.edu/labgc/est...
MJ Research Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.http://www.mjr.com/
Molecular Staging, Inc. Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT.http://www.molecularstaging.com/Pages...
Mutation Detection Systems - SSCP Analysis Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London, UK.http://www.ich.ucl.ac.uk/cmgs/sscp.htm
Nematode ITS1 Size Variation Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.http://nematode.unl.edu/its_id/EXAMPL...
Optimizing Multiplex and LA-PCR with Betaine LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.http://www-lecb.ncifcrf.gov/~pnh/pape...
Optimizing PCR Protocols Brief guidelines. From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/imr/optimize_pcr.html
PCR Amplification of cDNA Segments by 2 Stage Nested PCR Protocol from the method database of NIH, USA.http://www.ncbi.nlm.nih.gov/SNP/snp_v...
PCR Project Presentations from the University of California at Berkley on PCR, both current research reports and reviews.http://sunsite.berkeley.edu/biotech/pcr/
PCR Protocol Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
PCR Protocols Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State Universityhttp://www.cas.psu.edu/docs/CASDEPT/V...
PCR Technology An introduction by Connie Veilleux from the US National Health Museum website.http://www.accessexcellence.org/LC/SS...
PCR Troubleshooting Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.http://info.med.yale.edu/genetics/war...
PCR and multiplex PCR guide Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.http://www.info.med.yale.edu/genetics...
PCR animated Animation illustrating the principle of PCR, from the University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/pr...
PCR method protocols Protocols for PCR posted by the Helen Donis-Keller Laboratory.http://hg.wustl.edu/hdk_lab_manual/pc...
PCR primer design and reaction optimisation Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.http://web.uct.ac.za/microbiology/pcr...
PCR protocols Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School.http://genome.wustl.edu/tools/protoco...
Polymerase Chain Reaction Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.http://www.accessexcellence.com/RC/CT...
Web Source of PCR (1)
Legal Life Experience Degrees Get an Accredited & Verifiable US Biochemistry and Molecular Biology degree from home. Free Instant Delivery via Dhl. No study, no exams. Read Testimonials. www.belforduniversity.org (sponsored link) 3' RACE PCR This technique is used to obtain the 3''end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK.http://www.nottingham.ac.uk/~mbzspd/m...
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Adjuvants in PCR Reactions Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.http://info.med.yale.edu/genetics/war...
Alu-PCR Hybridization A report and protocol, from Fondation Jean Dausset.http://www.cephb.fr/manips_alupcr.html
Anchor Probes for Comparative Mapping of Grass Species Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.http://greengenes.cit.cornell.edu/anc...
Attotron Biosensor Corporation R & D company for development of biosensors and related products for the research and educational markets.http://www.attotron.com
Biosource molecular methods booklet 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).http://www.biosource.com/content/tech...
C. elegans Gene Knockout Project Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/
Cleaved Amplified Polymorphic Sequence Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.http://www.fsl.orst.edu/tgerc/PCRCour...
DNALC: PCR Animation An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.http://www.dnalc.org/shockwave/pcranw...
Degenerate PCR An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.http://cgat.ukm.my/protease/degpcr.html
Design Oligonunucleotide Primers Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.http://sciborg.uwaterloo.ca/~mpalmer/...
Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.http://imd-www.es.hokudai.ac.jp/~kinj...
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.http://www-ang.kfunigraz.ac.at/~binde...
Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Effect of PCR Buffer on multiplex PCR (PDF) Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)http://www.qiagen.com/literature/broc...
FISH Guide and Troubleshooting Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.http://info.med.yale.edu/genetics/war...
Fidelity of DNA Polymerases for PCR Article by PN Hengen from TIBS 1995http://www.lecb.ncifcrf.gov/~pnh/pape...
GenHunter Manufacturer of material for differential display PCR in Nashville, Tenn USA.http://www.genhunter.com
HiFi DNA HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.http://www.hifidna.com/
Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.http://www.btc-bti.com/pcrelisa.htm
Innovative Tools for Amplification Notes about PCR applications, principles, research results, from Roche.http://www.roche-applied-science.com/...
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Adjuvants in PCR Reactions Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.http://info.med.yale.edu/genetics/war...
Alu-PCR Hybridization A report and protocol, from Fondation Jean Dausset.http://www.cephb.fr/manips_alupcr.html
Anchor Probes for Comparative Mapping of Grass Species Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.http://greengenes.cit.cornell.edu/anc...
Attotron Biosensor Corporation R & D company for development of biosensors and related products for the research and educational markets.http://www.attotron.com
Biosource molecular methods booklet 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).http://www.biosource.com/content/tech...
C. elegans Gene Knockout Project Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/
Cleaved Amplified Polymorphic Sequence Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.http://www.fsl.orst.edu/tgerc/PCRCour...
DNALC: PCR Animation An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.http://www.dnalc.org/shockwave/pcranw...
Degenerate PCR An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.http://cgat.ukm.my/protease/degpcr.html
Design Oligonunucleotide Primers Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.http://sciborg.uwaterloo.ca/~mpalmer/...
Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.http://imd-www.es.hokudai.ac.jp/~kinj...
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.http://www-ang.kfunigraz.ac.at/~binde...
Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...
Effect of PCR Buffer on multiplex PCR (PDF) Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)http://www.qiagen.com/literature/broc...
FISH Guide and Troubleshooting Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.http://info.med.yale.edu/genetics/war...
Fidelity of DNA Polymerases for PCR Article by PN Hengen from TIBS 1995http://www.lecb.ncifcrf.gov/~pnh/pape...
GenHunter Manufacturer of material for differential display PCR in Nashville, Tenn USA.http://www.genhunter.com
HiFi DNA HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.http://www.hifidna.com/
Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.http://www.btc-bti.com/pcrelisa.htm
Innovative Tools for Amplification Notes about PCR applications, principles, research results, from Roche.http://www.roche-applied-science.com/...
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