Molecular Biology Laboratory Manual
CHAPTER 1: General Laboratory Methods
CHAPTER 2: Instructions for Notebook Keeping
CHAPTER 3: Vector NTI User's Guide
CHAPTER 4: Molecular Biology Methods
Preparation of genomic DNA from bacteria
Restriction enzyme digestion of DNA
Phenol/chloroform extraction of DNA
Transformation of E. coli by electroporation
Preparative DNA Fragment Isolation from an Agarose Gel
Ligations of plasmid DNA to insert DNA
Transfection of mammalian cells using Lipofectamine
Western Blot analysis of His-tagged proteinsl
Cycle Sequencing Protocols For ABI 3100
One Step Gene Assembly (Gene Synthesis)
CHAPTER 5: Tissue Culture Methods
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1 comment:
Instead of doing 'One Step Gene Assembly (Gene Synthesis)' on your own you are better of to order the final construct online! http://mrgene.com
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