Polymerase Chain Reaction (PCR)-2

PCR Primer Design Tools

PCR RFLP

PCR SSCP

PCR Troubleshooting

PCR, RT-PCR, and Real Time PCR Tutorials

Polymerase Chain Reaction (Molecular Info)

Polymerase chain reaction--PCR

RACE PCR

RAPD PCR

REAL TIME PCR Papers

Real Time PCR Protocols

Regular PCR Procedure

REP-PCR

RT PCR Protocols

Special PCR Protocols

Tail PCR

Tc1 Deletion PCR Tips: MGH

Touchdown PCR

Variants of PCR (1)

Variants of PCR (2)

Variants of PCR (3)

Variants of PCR (4)

Variants of PCR (5)

Vectorette PCR

Videos and Animations for PCR

Web Source of PCR (1)

Web Source of PCR (2)

Web Source of PCR (3)

Web Source of PCR (3)

Cellular Biology

Aseptic Cell Transfers

Calcium Phosphate tranfection of adherent mammalian cells (works well for 293 cells)

Cell mass by Measurement of Turbidity

Computation of Transfer Aliquots

Culturing MEFs
Calcium Phosphate Coprecipitation

Determining cell counts

Effectene (QIAGEN) tranfection of adherent mammalian cells (works well for HeLas and many other cell types)

Establishing stable cell lines

Establishment of a Primary Culture

Eukaryote Growth Dynamics

Examination of Bacterial Colonies

Fugene (Roche) tranfection of adherent mammalian cellsDOTAP (Roche) tranfection of adherent mammalian cells

Fusing cells to create heterokaryons

Gram Stain (+\-)

Harvesting murine bone marrow

Hematopoietic CFC assays

Maintenance

Metaphase chromosome preparation

Preservation and storage

Prokaryote Cell Number by Dilution Plating

Purification of L-KS+ marrow cells
Retrovirus Production
STO and ES Cell Culture

Safety considerations

Tissue culture methods

Tissue Culture of PtK1 Cells

Transfer of Eukaryote Suspension Cultures

TUNEL Protocol

Types of cells grown in culture

Viability Cell Count

Work area and equipment
XC assay of MoMLV

Cellular Organelle

MT/Organelle Motility Assay*

Membrane Mixes: Variations on a Theme*

Preparation of Rat Liver Cell Cytosol and/or Organelle Fractions*

Preparation of Sea Urchin Sperm Axonemes*

Preparation of Porcine Brain Tubulin*

Immunology

Antibody cleanup Yale Genome Analysis Center, Yale University

Bioengineering and Immunology (Video Protocol)

Computer immunology

Conjugation of monoclonal antibodies (complete) Dr. M Roederer, NIH, Vaccine Research Center

Direct Immunofluorescence Staining of Mononuclear Cells BD Biosciences

Direct Immunofluorescence Staining of Whole Blood Using Ammonium Chloride Lysing Solution BD Biosciences

Iindirect immunoflourescence for yeast I Dr. DE Koshland, Carnegie Institution of Washington

Immunofluorescence Protocols and Solutions

Immunofluorescence staining of cell suspensions of murine lymphoid tissue EBioscience.com

Immunofluorescence staining of human peripheral blood EBioscience.com

Immunofluorescence Technique Dr. DL Spector, Cold Spring Harbor Laboratory

Immunofluorescent Staining of Mouse and Rat Leucocytes BD Biosciences

Immunohistochemistry on Cryosections

Immunology Field Trip--Protocol

In Situ Probe

Indirect Immunofluorescence Staining of Mononuclear Cells BD Biosciences

Isolation of Resting B Cells From Mouse Spleen

MACS Depletion Drs. L Herzenberg, Stanford University

Making FITC and Biotin conjugates Therapeutic Immunology Group, Oxford University

Preparing cells for immunofluorescence Yale Genome Analysis Center, Yale University

Flow Cytometry (FCM)-1

7-AAD Staining of Dead Cells

A flow cytometric protocol for detection of Cryptosporidium spp.

Blocking FC-Receptor Binding

Calibrating Becton Dickinson Flow Cytometers for RFI Measurements

CD38 Fluorescence Intensity Quantitation

Detection of Apoptosis by 7-AAD Staining

DNA Staining for Cell Cycle Analysis

FCM: DNA Analysis by Flow Cytometry

FCM: FASC Analysis of Cell Cycle using BrdU and PI

FCM: Immunofluorescent Staining of Rat Thymocytes

FCM: Sodium and Potassium Analysis by Flow Cytometry

Flow Cytometry Glossary

Flow cytometry of murine blood

Flow Cytometry Principles （UC Berkeley）

Flow Cytometry Protocol

Flow Cytometry Protocol for Intracellular Staining Using Conjugated Second Antibody.

Flow Cytometry Protocol for Staining of Colonocytes - Surface Antigens

Flow Cytometry: Calcium Flux Analysis

Flow Cytometry: Membrane Potential Analysis by Flow Cytometry

Flow Cytometry：Annexin V Protocol

Flow Cytometry：CaspaTag Caspase Activity Protocol

Flow Cytometry：PARP PE

GENERAL CELL STAINING PROTOCOL FOR FLOW CYTOMETRY 1)

Immunofluorescent Staining for Flow Cytometry.

Indirect flow cytometry (FACS) protocol | Abcam

Intracellular Antigen Staining

Lineage analysis of blood
MEF nuclei prep for DNA content
Flow of surface and nuclear markers

Flow Cytometry (FCM)-2

BrdU and PI Staining Cells for Flow

Simultaneous BrdU and surface staining
Flow sorting fibroblasts for GFP
Setup and Use of the Cytometer
96-well Flow Setup Record (PDF)
Counting Fluorescently Stained Cells

Measurement of GFP Expression and DNA Content in Permeabilized Cells

Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells

Measurement of Intracellular CA2+ and Cell Surface Antigen Expression -- NEW!

Method for EtOH fixation of cells for long-term storage and DNA staining

Minimizing Background Staining

Monoclonal Antibody Staining Procedure

Preparation of 2% Formaldehyde Solution

Propidium Iodide Staining of Dead Cells

Protocol: Flow Cytometry (Millipore)

Protocols (BD Bioscience)

Questions and Answers about Cell Sorting

Simultaneous Measurement of Apoptosis and Necrosis

Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA content

Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content

Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content

Staining Procedure for Whole Blood

Standard Flow Cytometry Protocol

Surface Antigen Staining and DNA Content

Surface Staining for Cell Sorting

BIOCHEMISTRY

Cell Wall prep from Arabidopsis

GC analysis of alditol acetates

Golgi-Vesicles preparation from pea etiolated hypocotyls

High Voltage Paper Electrophoresis

Lipid extraction

Thin Layer Chromatography

Transport of UDP-[3H]-Glc to Golgi-Vesicles

UDP-sugar binding assay

Resources of Biochemical Pathways